Journal: Cell reports

Article Title: Modulation of Peptidoglycan Synthesis by Recycled Cell Wall Tetrapeptides

doi: 10.1016/j.celrep.2020.107578

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Penta-His antibody QIAGEN Cat#34660; RRID:AB_2619735 Anti-Mouse IgG Sigma Cat#A9044; RRID:AB_258431 Bacterial and Virus Strains All bacterial strains are listed in Table S1 This study Table S1 Chemicals, Peptides, and Recombinant Proteins D-Methionine Sigma Cat#M9375 L-Methionine Merck Cat#105707 Critical Commercial Assays Ni-NTA Agarose QIAGEN Cat#30210 Bio-Rad Protein Assay Dye Reagent Concentrate Bio-Rad Cat#500-0006 GeneJET Genomic DNA Purification Kit Thermo Fisher Scientific Cat#K0721 Qubit® dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q32851 Nextera XT DNA Library Preparation Kit Illumina Cat#FC-131-1024 MiSeq Reagent Kit v2 (50-cycles) Illumina Cat#MS-102-2001 Deposited Data Tn-seq experiments_Raw data This study https://www.ncbi.nlm.nih.gov BioProject ID: PRJNA623082 WGS suppressor mutants_Raw data This study https://www.ncbi.nlm.nih.gov BioProject ID: PRJNA623074 Oligonucleotides All oligonucleotides are detailed in Tables S2 – S4 This study Tables S2 – S4 Recombinant DNA pET28b:: vc2153 -6xHis This study FC281 pET28b:: ldcA -6xHis This study FC2025 pET22b:: vca0337 -6xHis This study FC2662 pET28b:: slt70 -6xHis ( E. coli ) Espaillat et al., 2016 FC1860 pET28b:: ldtA -6xHis Cava et al., 2011 FC1168 pET28b::AHA1477-6xHis This study FC2772 pET28b::PMI1557-6xHis This study FC2773 pET28b::STM1800-6xHis This study FC2774 pBAD 33 :: ldcA- 6xHis This study FC2753 pBAD 18-Km :: ldtA- 6xHis This study FC2759 pBAD 18-Km :: murA- 6xHis This study FC2838 pBAD 18-Km :: murB- 6xHis This study FC2839 pBAD 18-Km :: murC- 6xHis This study FC2840 pBAD 18-Km :: murE- 6xHis This study FC2841 pBAD 18-Km :: murF- 6xHis This study FC2842 Software and Algorithms GraphPad Prism GraphPad Software, Inc. https://www.graphpad.com Zeiss Zen Blue Software Zeiss https://www.zeiss.com/corporate/int/home.html ImageJ https://imagej.nih.gov/ij/ https://imagej.nih.gov/ij/ NCBI blast https://www.ncbi.nlm.nih.gov/BLAST/ https://www.ncbi.nlm.nih.gov/BLAST/ Galaxy server tools Afgan et al., 2016 https://usegalaxy.org/ Open in a separate window KEY RESOURCES TABLE.

Techniques: Virus, Recombinant, DNA Purification, DNA Library Preparation, Software

Journal: Cancer cell

Article Title: Vitamin B6 addiction in acute myeloid leukemia

doi: 10.1016/j.ccell.2019.12.002

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse AML cell lines, human AML cell lines, iMEF cell line, 3T3 cell line and human sarcoma cell line were infected with virus encoding Cas9 (Addgene 52962) followed with blastcitidin selection to generate Cas9 cell lines.

Techniques: Plasmid Preparation, Recombinant, Mutagenesis, RNA Extraction, Gel Extraction, Clone Assay, Purification, Flow Cytometry, Sequencing, CRISPR, Functional Assay, shRNA, Software